The Pyronema confluens genome page at the Ruhr-University Bochum

Pyronema confluens is a filamentous ascomycete from the early-diverging group of Pezizales. It is one of the few members of this group that easily produces fruiting bodies (sexual structures) under laboratory conditions, and was one of the model fungi early in the 20th century that were used to elucidate the dikaryotic stage during sexual development of ascomycetes. In recent years, we have performed several small-scale comparative studies of fungal multicellular development with P. confluens and higher filamentous ascomycetes. These studies already showed that gene expression is conserved to a relatively large degree even in distantly related ascomycete groups. To address the question of the evolution of fungal multicellular development at a larger scale, we have now sequenced the genome and transcriptomes from several different developmental stages of P. confluens.
The nuclear genome was assembled from a combination of Illumina/Solexa and 454 reads. Gene models were predicted de novo based on homology and evidence from the RNA-seq data. The mitochondrial genome was assembled separately from Illumina, 454, and PacBio reads. The following table gives a summary of key features of the P. confluens genome:

nuclear genome assembly size	50 Mb
number of scaffolds	1588
scaffold N50	135 kb
protein coding genes	13369
average size of CDSs	1093 nt
average size of mRNAs	1483 nt
tRNA genes	605
% coding sequence	29.2
size of mtDNA	191 kb

More information is available in the following publications:

Traeger S, Altegoer F, Freitag M, Gabaldon T, Kempken F, Kumar A, Marcet-Houben M, Pöggeler S, Stajich JE, Nowrousian M (2013) The genome and development-dependent transcriptomes of Pyronema confluens: a window into fungal evolution. PLoS Genet 9: e1003820. Paper at PLoS Genetics pdf

Nowrousian M (2016) Complete mitochondrial genome sequence of the Pezizomycete Pyronema confluens. Genome Announc 4: e00355-16. Paper at Genome Announcements pdf

The Pyronema confluens genome homepage at the Ruhr-University Bochum